Mouse cloning and somatic cell reprogramming using electrofused blastomeres
نویسندگان
چکیده
منابع مشابه
Reprogramming after Chromosome Transfer into Mouse Blastomeres
It is well known that oocytes can reprogram differentiated cells, allowing animal cloning by nuclear transfer. We have recently shown that fertilized zygotes retain reprogramming activities, suggesting that such activities might also persist in cleavage-stage embryos. Here, we used chromosome transplantation techniques to investigate whether the blastomeres of two-cell-stage mouse embryos can r...
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Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
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With the help of the technique of Sendai virus-mediated cell fusion, hybrid cells were produced between two maturing oocytes, between maturing oocytes or mature secondary oocytes and interphase blastomeres from 2-cell embryos, and between secondary oocytes and follicle cells (FC). In the first case giant oocytes form and in these the two groups of condensing bivalents join on a common spindle, ...
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ژورنال
عنوان ژورنال: Cell Research
سال: 2010
ISSN: 1001-0602,1748-7838
DOI: 10.1038/cr.2010.180